Nuclear Size Scaling during Xenopus Early Development Contributes to Midblastula Transition Timing
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منابع مشابه
Nuclear Size Scaling during Xenopus Early Development Contributes to Midblastula Transition Timing
Early Xenopus laevis embryogenesis is a robust system for investigating mechanisms of developmental timing. After a series of rapid cell divisions with concomitant reductions in cell size, the first major developmental transition is the midblastula transition (MBT), when zygotic transcription begins and cell cycles elongate. Whereas the maintenance of a constant nuclear-to-cytoplasmic (N/C) vol...
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Dramatic changes in cell and nuclear size occur during development and differentiation, and aberrant nuclear size is associated with many disease states. However, the mechanisms that regulate nuclear size are largely unknown. A robust system for investigating nuclear size is early Xenopus laevis development, during which reductions in nuclear size occur without changes in DNA content. To identi...
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Transcripts from the Xenopus gene GS17 are absent from oocytes and first appear at the midblastula transition (MBT), about 8 hr after fertilization. Injection experiments with deletions of the GS17 gene and GS17-beta-globin hybrid genes show that a relatively short sequence of 74 base pairs is sufficient to activate, in cis, transcription at the MBT. This 74-base sequence is located about 700 b...
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Xenopus embryos (UV embryos) resulting from u.v. (254 nm) irradiation to the vegetal egg hemisphere and thus developing little or no axial pattern (UV5-Scharf & Gerhart, 1983), have been compared histologically with synchronous normal siblings at each of three stages. In addition, the relative amounts of blood-forming tissue produced in normal and in UV embryos have been studied by Western blot...
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The first 12 cleavage divisions in Xenopus embryos provide a natural experiment in size scaling, as cell radius decreases ∼16-fold with little change in biochemistry. Analyzing both natural cleavage and egg extract partitioned into droplets revealed that mitotic spindle size scales with cell size, with an upper limit in very large cells. We discuss spindle-size scaling in the small- and large-c...
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ژورنال
عنوان ژورنال: Current Biology
سال: 2015
ISSN: 0960-9822
DOI: 10.1016/j.cub.2014.10.051